Removal of albumin from multiple human serum samples.

were assayed for recall proliferative response to OVA 323-339. Emulsions prepared by either the dual-syringe or sonification method stimulated a strong T-cell response, as demonstrated by the ex vivo proliferation assay. A likelihood ratio test indicated a stronger immunization with the sonified emulsion transferred by the method outlined above than with the emulsion prepared by the dual-syringe technique (P = 0.024). Thus, the sonification technique produces an effective emulsion in a fraction of the time required by repeated passage between linked syringes, and the transfer technique outlined here renders this sonification easier and less wasteful.